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In distinction to random primers, oligo (dT) primers are usually not well suited for degraded samples but are outstanding for constructing cDNA libraries and advised to be used with reverse transcriptases (RTs) as Superscript IV RT.Formulated being temperature insensitive, it is possible to rely on them whether or not the ailments are sub-zero or s

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As opposed to oligo(dT) primers, these primers are usually not template unique and are appropriate for DNA synthesis applying Klenow fragments with DNA templates or for cDNA synthesis utilizing reverse transcriptase with mRNA templates.Swimming pools of pre-created primers might help generate comprehensive expression profiles, Particularly wherever